lQuicking Deoxynivalenol (Vomitoxin) Rapid Test enables the detection limit of 200 ppb.
lQuicking Deoxynivalenol (Vomitoxin) Rapid Test is a quick and easy testing tool that can be performed in just 10 minutes.
lQuicking Deoxynivalenol (Vomitoxin) Rapid Test can be stored for 18 months at room temperature, between 2 and 30°C.
- 10×foil pouches each contains one DON cassette with a pipette and a desiccant
- 1×concentrated assay diluent (8 mL)
- 2×centrifugal tube (15 mL)
- 10×centrifugal tube (1.5 mL)
- Products Manual
-Assay diluent preparation: dilute 20× concentrate with distilled water (i.e. 1 mL + 19 mL). In case of precipitates, bring the diluents to room temperature and mix well into solution.
-Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 1.0 g ground portion of the sample into the centrifugal tube (15 mL).
-Add assay diluent into the tube and cover the lid. Shake for 5 min and do a centrifugation at 4000 rpm for 1 min to get supernatant. NOTE: Please refer to below table for the amount of applied assay diluent. (REF: Table )
Assay Diluents (mL)
-Transfer 0.2 mL of supernatant into a 1.5 mL centrifugal tube. Add 0.8 mL of assay diluent into the tube and mix well. Use the mixture for the assay.
-Take out the cassette from the foil pouch and place it horizontally.
-Suck the liquid and drop 3 drops into the assay sample hole “S”.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Interpretation of results
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Deoxynivalenol is at or above 200 ppb in the samples.
Negative: The presence of both bands in C zone and T zone.